| 产品编号 | bs-6156R |
| 英文名称 | RASGEF1A Rabbit pAb |
| 中文名称 | 鸟嘌呤核苷酸交换因子rasgef1a抗体 |
| 别 名 | CG4853 gene product; Ras-GEF domain-containing family member 1A; RasGEF domain family, member 1A; rasgef1a; RGF1A_HUMAN; RASGEF 1A; RASGEF1A. |
| 研究领域 | 肿瘤 信号转导 细胞凋亡 转录调节因子 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RASGEF1A: 301-200/481 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration. Tissue Specificity: Detected in brain and spinal cord. Highly expressed in a number of intrahepatic cholangiocarcinoma tissue biopsies. Similarity: Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. SWISS: Q8N9B8 Gene ID: 221002 Database links: Entrez Gene: 221002 Human Entrez Gene: 70727 Mouse SwissProt: Q8N9B8 Human Unigene: 125293 Human Unigene: 87660 Mouse Unigene: 29825 Rat |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RASGEF1A Polyclonal Antibody, Unconjugated(bs-6156R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
