| 产品编号 | bs-6225R |
| 英文名称 | CAMK1D Rabbit pAb |
| 中文名称 | 钙调蛋白激酶CaMK1D抗体 |
| 别 名 | CKLiK; CaM-K1; CaMKID; A630059D12Rik; CaMKIdelta; E030025C11Rik; mCKLiK; RGD1560691; KCC1D_HUMAN; CAMK1D; CaM kinase I delta (CaM kinase ID | CaM-KI delta | CaMKI delta | CaMKID); CaMKI-like protein kinase (CKLiK); 2.7.11.17; KCC1D_MOUSE; CaM kinase I del |
| 研究领域 | 肿瘤 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CAMK1D: 33-130/385 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CAMK1D is a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. It may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway and may play a role in apoptosis of erythroleukemia cells. Function: Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta. Subcellular Location: Cytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Also nuclear upon activation. Tissue Specificity: Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. SWISS: Q8IU85 Gene ID: 57118 Database links: Entrez Gene: 57118 Human Entrez Gene: 227541 Mouse Omim: 607957 Human SwissProt: Q8IU85 Human SwissProt: Q8BW96 Mouse Unigene: 659517 Human Unigene: 191949 Mouse Unigene: 200021 Rat |
| 产品图片 |
Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CAMK1D Polyclonal Antibody, Unconjugated(bs-6225R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
