| 产品编号 | bs-6563R |
| 英文名称 | NMT1 Rabbit pAb |
| 中文名称 | 豆蔻酰基转移酶1抗体 |
| 别 名 | HsNMT1; NMT; NMT1_HUMAN; NMT1; Myristoyl-CoA:protein N-myristoyltransferase 1 (HsNMT1 | NMT 1 | Type I N-myristoyltransferase); Peptide N-myristoyltransferase 1; Protein-lysine myristoyltransferase NMT1; 2.3.1.97; |
| 研究领域 | 肿瘤 细胞生物 干细胞 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NMT1: 231-330/496 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) Function: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Subcellular Location: Cytoplasm. Tissue Specificity: Heart, gut, kidney, liver and placenta. Similarity: Belongs to the NMT family. SWISS: P30419 Gene ID: 4836 Database links: Entrez Gene: 4836 Human Omim: 160993 Human SwissProt: P30419 Human Unigene: 532790 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |