产品编号 | bs-7542R |
英文名称 | Serine carboxypeptidase 1 Rabbit pAb |
中文名称 | 丝氨酸羧肽酶1抗体 |
别 名 | HSCP 1; HSCP1; likely homolog of rat and mouse retinoid-inducible serine carboxypeptidase; Retinoid inducible serine carboxypeptidase; Retinoid-inducible serine carboxypeptidase precursor; SCP 1; SCP1; SCPEP 1; SCPEP1; Serine carboxypeptidase 1; serine carboxypeptidase 1 precursor protein; RISC_HUMAN. |
研究领域 | 心血管 细胞生物 免疫学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human, (predicted: Mouse, Rat, ) |
产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 48 kDa |
检测分子量 | |
细胞定位 | 细胞浆 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human RISC/Serine carboxypeptidase 1: 121-320/452 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Vascular smooth muscle activation is a salient feature of several pathological conditions including atherosclerosis, hypertension, vein graft failure, restenosis, and transplant arteriopathy. Several new genes whose proteins could lead to such biological effects have been identified. One such protein is RISC. Tissue expression pattern of Retinoid-inducible serine carboxypeptidase (RISC) in human revealed that it was highly expressed in kidney and heart. Function: May be involved in vascular wall and kidney homeostasis (By similarity). Subcellular Location: Secreted (Potential). Similarity: Belongs to the peptidase S10 family. SWISS: Q9HB40 Gene ID: 59342 Database links: Entrez Gene: 59342 Human Entrez Gene: 74617 Mouse SwissProt: Q9HB40 Human SwissProt: Q920A5 Mouse Unigene: 514950 Human Unigene: 34126 Mouse |
产品图片 |
Tissue/cell: Human kidney; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RISC/Serine carboxypeptidase 1 Polyclonal Antibody, Unconjugated(bs-7542R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |