| 产品编号 | bs-6768R |
| 英文名称 | PEF1 Rabbit pAb |
| 中文名称 | 调亡诱导因子家族蛋白PEF1抗体 |
| 别 名 | ABP32; PEF; PEF protein with a long N terminal hydrophobic domain; PEF1A; PEFLIN; Penta EF hand domain containing protein 1. |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 转录调节因子 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Pig, Cow, Horse, Sheep, Monkey, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PEF1: 121-230/284 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PEF1 (penta-EF hand domain containing 1) is a Ca(2+)-binding protein that belongs to the penta-EF hand family which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca(2+). Subunit: Heterodimer; heterodimerizes with PDCD6. Dissociates from PDCD6 in presence of Ca(2+). Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+). Similarity: Contains 5 EF-hand domains. SWISS: Q9UBV8 Gene ID: 553115 Database links: Entrez Gene: 553115 Human Entrez Gene: 67898 Mouse Omim: 610033 Human SwissProt: Q9UBV8 Human SwissProt: Q8BFY6 Mouse Unigene: 470417 Human Unigene: 20818 Mouse Unigene: 96097 Rat |
| 产品图片 |
Sample: Intestine (Mouse) Lysate at 40 ug
Primary: Anti-PEF1 (bs-6768R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
