| 产品编号 | bs-6793R |
| 英文名称 | APPD Rabbit pAb |
| 中文名称 | 凋亡诱导蛋白D抗体 |
| 别 名 | PKHF1_HUMAN; Apoptosis inducing protein D; LAPF; Lysosome associated apoptosis inducing protein containing PH and FYVE domains; MGC4090; PH and FYVE domain containing protein 1; PH domain containing family F member 1; Phafin 1; Pleckstrin homology domain containing family F(with FYVE domain) member 1; Pleckstrin homology domain containing family F member 1; Pleckstrin homology domain containing family F with FYVE domain member 1; PLEKHF1; ZFYVE15; Zinc finger FYVE domain containing protein 15. |
| 研究领域 | 细胞生物 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APPD/LAPF: 75-124/279 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
APPD is a lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF). It is a representative of a novel family of PH and FYVE domain-containing proteins and induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway.
Cellular localization: Nucleus. Cytoplasm, perinuclear region. Lysosome. Note=Translocates to lysosome during apoptosis. Function: May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8. Subcellular Location: Nucleus. Cytoplasm, perinuclear region. Lysosome. Note=Translocates to lysosome during apoptosis. Tissue Specificity: Highly expressed in heart and skeletal muscle. Weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. Similarity: Contains 1 FYVE-type zinc finger. Contains 1 PH domain. SWISS: Q96S99 Gene ID: 79156 Database links: Entrez Gene: 79156 Human Entrez Gene: 72287 Mouse SwissProt: Q96S99 Human SwissProt: Q3TB82 Mouse Unigene: 466383 Human Unigene: 58389 Human Unigene: 333798 Mouse Unigene: 9380 Rat |
| 产品图片 |
Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APPD Polyclonal Antibody, Unconjugated(bs-6793R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APPD Polyclonal Antibody, Unconjugated(bs-6793R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
