| 产品编号 | bs-7566R |
| 英文名称 | RHBDD3 Rabbit pAb |
| 中文名称 | 垂体瘤细胞凋亡抗体 |
| 别 名 | C22orf3; HS984G1A; Pituitary tumor apoptosis; PTAG; RHBD3_HUMAN; RHBDD3; Rhomboid domain containing 3; Rhomboid domain-containing protein 3. |
| 研究领域 | 细胞生物 神经生物学 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RHBDD3: 151-250/386 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PTAG is a novel 386 amino acid multi-pass membrane protein that contains one UBA domain and augments drug-induced apoptosis. Cells lacking PTAG have a reduced apoptotic response, thereby causing a predisposition to cell malignancy and resistance to chemotherapeutic interventions, and PTAG plays a role in colorectal tumorigenesis as the majority of primary colorectal tumors lack the PTAG gene. Encoded by a gene located on human chromosome 22, PTAG is often co-expressed with EWS (ewing sarcoma breakpoint region 1), a gene located directly downstream of PTAG. Subcellular Location: Membrane; Multi-pass membrane protein (Potential). Similarity: Contains 1 UBA domain SWISS: Q9Y3P4 Gene ID: 25807 Database links: Entrez Gene: 25807 Human Entrez Gene: 279766 Mouse SwissProt: Q9Y3P4 Human SwissProt: Q8BP97 Mouse Unigene: 106730 Human Unigene: 260866 Mouse Unigene: 9606 Rat RHBDD3分子是一个全新的TLR信号途径的负向调控蛋白分子,是一个能够显著抑制自身免疫性疾病发生的效应分子,RHBDD3控制机体避免发生过度的免疫应答、防止自身免疫性疾病的发生具有重要意义。 |
| 产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Placenta (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Skeletal muscle (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti- RHBDD3 (bs-7566R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33’51 kD
Observed band size: 51 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
