| 产品编号 | bs-7075R |
| 英文名称 | phospho-PBP (Ser153) Rabbit pAb |
| 中文名称 | 磷酸化Raf激酶抑制蛋白抗体 |
| 别 名 | PBP(phospho S153); p-PBP(phospho S153); p-RKIP(S153); p-RKIP(Ser153); RKIP(phospho-Ser153); RKIP(phospho-S153); phosphatidylethanolamine-binding protein; PEBP; PBP; Phosphatidylethanolamine-binding protein 1; PEBP-1; PEBP1; Prostatic-binding protein; Pros |
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Specific References (1) | bs-7075R has been referenced in 1 publications.
111
[IF=6.208] Viviana Tritto. et al. Changes of RAS Pathway Phosphorylation in Lymphoblastoid Cell Lines from Noonan Syndrome Patients Carrying Hypomorphic Variants in Two NS Genes. INT J MOL SCI. 2023 Jan;24(4):4035 WB ; Human. 222
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| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Mouse, Pig, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human RKIP around the phosphorylation site of Ser153: VA(p-S)FR |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
Function: Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. Subunit: Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Subcellular Location: Cytoplasm. Similarity: Belongs to the phosphatidylethanolamine-binding protein family. SWISS: P30086 Gene ID: 5037 Database links: Entrez Gene: 5037 Human Omim: 604591 Human SwissProt: P30086 Human Unigene: 433863 Human Unigene: 29745 Rat Raf激酶抑制蛋白(PKIP)属于磷脂酰乙醇胺结合蛋白（phosphatidylethanolamine-binding protein, PEBP）家族,广泛存在于各种不同的生物中,PKIP还参与了对NF-kB信号通路和G蛋白偶联受体信号通路的调控,参与NF-κB信号通路的调控,参与了对细胞内多种信号转导通路的调节作用。RKIP在膜的生物合成、精子发生、神经发育和细胞凋亡等生理过程中发挥重要作用，并参与了老年痴呆症及糖尿病等的病理过程。有学者认为：RKIP有抑制肿瘤细胞转移的作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
