| 产品编号 | bs-6771R |
| 英文名称 | ERN2 Rabbit pAb |
| 中文名称 | 内质网核信号转导蛋白2抗体 |
| 别 名 | ER to nucleus signalling 2; ERN 2; hIRE2p; Inositol requiring 1 beta; Ire1 beta; IRE1 S. cerevisiae homolog of; IRE2; Serine/threonine protein kinase/endoribonuclease IRE2; ERN2_HUMAN; Endoplasmic reticulum-to-nucleus signaling 2; Inositol-requiring protein 2 (hIRE2p); Ire1-beta (IRE1b). |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ERN2: 465-550/926 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ERN2, endoplasmic reticulum to nucleus signaling 2, induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. It is pro-apoptotic and appears to play no role in the unfolded-protein response, unlike closely related proteins. Function: Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Post-translational modifications: Autophosphorylated. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain. Database links: Entrez Gene: 10595 Human Entrez Gene: 26918 Mouse Omim: 604034 Human SwissProt: O75460 Human SwissProt: Q9EQY0 Mouse Unigene: 133982 Human Unigene: 20452 Mouse |
| 产品图片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-ERN2 antibody (bs-6771R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
