| 产品编号 | bs-7109R |
| 英文名称 | PARP4 Rabbit pAb |
| 中文名称 | 蛋白质单ADP核糖基转移酶PARP4抗体 |
| 别 名 | PARP related/IalphaI related H5/proline rich; Vault poly ADP ribose polymerase; VPARP; 193kDa vault protein; ADP ribosyltransferase(NAD+poly(ADP ribose) polymerase) like 1; ADP ribosyltransferase like 1; ADPRT L1; ADPRTL 1; ADPRTL1; H5 proline rich; I alpha I related; KIAA0177; Minor vault p193 protein; p193 antibody PARP 4; PARP related; PARPL; PH 5P; PH5P; Poly(ADP ribose) polymerase 4; Poly(ADP ribose) polymerase family member 4; Poly(ADP ribose) synthetase; Poly(ADP ribosyl) transferase like 1; Poly ADP ribose polymerase 4; Poly ADP ribose polymerase family member 4; Vault 3; Vault; Vault poly(ADP ribose) polymerase; Vault protein 193 kDa; Vault3; PARP4_HUMAN. |
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Specific References (2) | bs-7109R has been referenced in 2 publications.
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[IF=8.44] Lu Bingfeng. et al. C/D box small nucleolar RNA SNORD104 promotes endometrial cancer by regulating the 2ʹ-O-methylation of PARP1. J TRANSL MED. 2022 Dec;20(1):1-14 WB ; Human. 222
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[IF=1.12] Liu et al. Keeping the heart empty and beating: an alternative technique to preserve hypertrophied hearts during valvular surgery. (2015) J.Cardiothorac.Surg. 10:71 WB ; Porcine. 222
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| 研究领域 | 细胞生物 细胞凋亡 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Pig, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 192 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PARP4: 851-950/1724 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Component of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas. Subunit: Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Also found in the nucleus, associated with mitotic spindles. Tissue Specificity: Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas. Similarity: Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 1 VIT domain. [SIMILARITY] Contains 1 VWFA domain. SWISS: Q9UKK3 Gene ID: 143 Database links: Entrez Gene: 143 Human Omim: 607519 Human SwissProt: Q9UKK3 Human Unigene: 117825 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP4) Polyclonal Antibody, Unconjugated (bs-7109R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
