| 产品编号 | bs-7836R |
| 英文名称 | DCTN2 Rabbit pAb |
| 中文名称 | 动力蛋白激活蛋白2抗体 |
| 别 名 | 50 kD dynein associated polypeptide; 50 kDa dynein associated polypeptide; 50 kDa dynein-associated polypeptide; DCTN-50; DCTN2; DCTN2_HUMAN; DCTN50; dynactin 2(p50); dynactin 2; dynactin complex 50 kD subunit; Dynactin complex 50 kDa subunit; Dynactin su |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞周期蛋白 细胞分化 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dynamitin/DCTN2: 65-150/401 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Function: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Subunit: Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 and CEP135. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Membrane; Peripheral membrane protein. Similarity: Belongs to the dynactin subunit 2 family. SWISS: Q13561 Gene ID: 10540 Database links: UniProtKB/Swiss-Prot: Q13561.4 Entrez Gene: 10540 Human Entrez Gene: 69654 Mouse Omim: 607376 Human SwissProt: Q13561 Human SwissProt: Q99KJ8 Mouse Unigene: 289123 Human Unigene: 167537 Mouse |
| 产品图片 |
Sample:
Spleen (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti- DCTN2 (bs-7836R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- DCTN2 (bs-7836R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
