产品编号 | bs-8013R |
英文名称 | ITGB1BP2 Rabbit pAb |
中文名称 | 整合素β1结合蛋白2抗体 |
别 名 | CHORDC3; Integrin beta 1 binding protein(melusin) 2; integrin beta 1 binding protein 2; ITGB1BP; MELUSIN; MGC119214; ITGB1BP2/CHORDC3; MSTP015; ITBP2_HUMAN. |
研究领域 | 心血管 细胞生物 细胞粘附分子 细胞骨架 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 38 kDa |
检测分子量 | |
细胞定位 | 细胞核 细胞浆 细胞外基质 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ITGB1BP2/CHORDC3: 25-110/347 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
CHORDC3 is a muscle-specific protein that interacts with the Integrin 1 cytoplasmic domain, in the hypertrophic response to mechanical overload. Function: May play a role during maturation and/or organization of muscles cells. Subunit: Interacts with beta-1 integrin subunit. This interaction is regulated by divalent cations, and it occurs only in absence of calcium. Tissue Specificity: Expressed in skeletal and cardiac muscles but not in other tissues. Similarity: Contains 2 CHORD domains. Contains 1 CS domain. SWISS: Q9UKP3 Gene ID: 26548 Database links: Entrez Gene: 26548 Human Entrez Gene: 26549 Mouse Omim: 300332 Human SwissProt: Q9UKP3 Human SwissProt: Q9R000 Mouse Unigene: 109999 Human Unigene: 46232 Mouse |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ITGB1BP2) Polyclonal Antibody, Unconjugated (bs-8013R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ITGB1BP2) Polyclonal Antibody, Unconjugated (bs-8013R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |