| 产品编号 | bs-8034R |
| 英文名称 | COMMD1 Rabbit pAb |
| 中文名称 | 铜代谢结构域蛋白1抗体 |
| 别 名 | C2orf5; MURR1; U2/Mu; COMD1_HUMAN; COMMD1; Protein Murr1; COMD1_MOUSE; |
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Specific References (1) | bs-8034R has been referenced in 1 publications.
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[IF=3.541] Lili Zhang. et al. Disruption of COMMD1 accelerates diabetic atherosclerosis by promoting glycolysis. DIABETES VASC DIS RE. ;(): WB ; Mouse. 222
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| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COMMD1/MURR1: 95-190/190 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes. Tissue specificity: Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Function: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes. Subunit: Monomer and homodimer. Interacts (via COMM domain) with COMMD2, COMMD3, COMMD4, COMMD5, COMMD6, COMMD7, COMMD8 and COMMD10 (via COMM domain). Identified in a complex with an E3 ubiquitin ligase complex composed of TCEB1/elongin C, CUL2, SOCS1 and RBX1. Interacts directly with SOCS1 and CUL2. Interacts directly the N-terminal region of ATP7B. Interacts with CCS, CDKN2A, RELA and NFKBIB. Identified in a complex with NF-kappa-B. Interacts with CLU. Tissue Specificity: Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Post-translational modifications: Ubiquitinated; undergoes both 'Lys-63'- and 'Lys-48'-linked polyubiquitination. Ubiquitinated by XIAP, leading to its proteasomal degradation. Similarity: Contains 1 COMM domain. SWISS: Q8N668 Gene ID: 150684 Database links: Entrez Gene: 150684 Human Entrez Gene: 17846 Mouse Omim: 607238 Human SwissProt: Q8N668 Human SwissProt: Q8K4M5 Mouse Unigene: 468702 Human Unigene: 259903 Mouse Unigene: 451238 Mouse COMMD蛋白家族中最典型的成员.它是一个多效性因子,参与许多生理活动,包括铜代谢、钠运输、对核因子κB(NF-κB)及低氧诱导因子1(HIF-1)的调节等，COMMD广泛存在于多细胞生物中,最典型的特征是它们的羧基端存在一个高度保守而独特的结构—COMMD结构域,为蛋白质间的相互作用提供了关键界面. 目前研究COMMD蛋白家族有10个成员,即COMMD1～10.COMMD1是COMMD蛋白家族中最先被证实且研究最为深入的蛋白质,在不同物种中广泛表达,在人类的不同组织表达存在差异. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COMMD1) Polyclonal Antibody, Unconjugated (bs-8034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
4% Paraformaldehyde-fixed HepG2(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (COMMD1) polyclonal Antibody, unconjugated (bs-8034R) 1:50, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
