| 产品编号 | bs-8168R |
| 英文名称 | GTF2B Rabbit pAb |
| 中文名称 | 通用转录因子GTF2B抗体 |
| 别 名 | TF2B; TFIIB; TF2B_HUMAN; GTF2B; General transcription factor TFIIB; S300-II; 2.3.1.48; TF2B_MOUSE; RNA polymerase II alpha initiation factor; TF2B_RAT; |
| 研究领域 | 细胞生物 免疫学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GTF2B corresponding to amino acids 131-180.: 141-250/316 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Function: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Subunit: Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 (By similarity). Interacts with Epstein-Barr virus EBNA2. Subcellular Location: Nucleus Similarity: Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger. SWISS: Q00403 Gene ID: 2959 Database links: Entrez Gene: 2959 Human Entrez Gene: 229906 Mouse Omim: 189963 Human SwissProt: Q00403 Human SwissProt: P62915 Mouse Unigene: 481852 Human Unigene: 271756 Mouse Unigene: 6109 Rat |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- GTF2B (bs-8168R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
