| 产品编号 | bs-8177R |
| 英文名称 | KIF11 Rabbit pAb |
| 中文名称 | 驱动蛋白样蛋白1/甲状腺激素受体相互作用蛋白5抗体 |
| 别 名 | HKSP; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; KNSL1; Eg5; Thyroid receptor interacting protein 5; TRIP5; KIF11_HUMAN. |
 |
Specific References (1) | bs-8177R has been referenced in 1 publications.
111
[IF=4.86] Junjun Ling. et al. KIF11, a plus end-directed kinesin, as a key gene in benzo(a)pyrene-induced non-small cell lung cancer. Environ Toxicol Phar. 2022 Jan;89:103775 IHC ; Human. 222
|
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 细胞骨架 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 120 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIF11/Eg5: 351-450/1056 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Eg5 is a motor protein that belongs to the kinesin-like family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Function: Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. Subunit: Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Post-translational modifications: Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR. A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. Similarity: Belongs to the kinesin-like protein family. BimC subfamily. Contains 1 kinesin-motor domain. SWISS: P52732 Gene ID: 3832 Database links: Entrez Gene: 3832 Human Omim: 148760 Human SwissProt: P52732 Human Unigene: 8878 Human |
| 产品图片 |
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-KIF11 (bs-8177R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KIF11 Polyclonal Antibody, Unconjugated(bs-8177R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
