| 产品编号 | bs-8386R |
| 英文名称 | Ecadherin binding protein E7 Rabbit pAb |
| 中文名称 | 上皮钙粘附分子结合蛋白E7 |
| 别 名 | HAKAI; RNF188; c-Cbl-like; HAKAI_HUMAN; CBLL1; Casitas B-lineage lymphoma-transforming sequence-like protein 1 (c-Cbl-like protein 1); RING finger protein 188; RING-type E3 ubiquitin transferase Hakai; 2.3.2.27; HAKAI_MOUSE; E-cadherin binding protein E7; |
 |
Specific References (2) | bs-8386R has been referenced in 2 publications.
111
[IF=5.17] Zhang et al. Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/β-catenin pathway. (2015) Oncotarge. 6:3123-35
111
[IF=1.664] Zhang PF et al. MicroRNA-139 suppresses hepatocellular carcinoma cell proliferation and migration by directly targeting Topoisomerase I. ONCOLOGY LETTERS 17: 1903-1913, 2019 WB ; Human. 222
|
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CBLL1/Ecadherin binding protein E7: 221-320/491 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CBLL1, also known as HAKAI (meaning ‘destruction’ in Japanese), or RNF188 (RING finger protein 188), is a 491 amino acid protein that contains one C2H2-type zinc finger and one RING-type zinc finger. CBLL1 is believed to function as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation. More specifically, upon activation of c-Src, CBLL1 interacts with and ubiquitinates tyrosine-phosphorylated E-cadherin, thereby targeting the E-cadherin complex for endocytosis and disrupting epithelial cell-cell contacts. Via its role as an E-cadherin regulator, CBLL1 participates in cell adhesion and may also be involved in the regulation of epithelial-mesenchymal transitions. Function: Promotes ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1. Targets CDH1 for endocytosis and degradation (By similarity). Subunit: Homodimer. Interacts with tyrosine-phosphorylated SRC substrates. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 RING-type zinc finger. SWISS: Q75N03 Gene ID: 79872 Database links: Entrez Gene: 79872 Human Entrez Gene: 104836 Mouse Omim: 606872 Human SwissProt: Q75N03 Human SwissProt: Q9JIY2 Mouse Unigene: 592271 Human Unigene: 273270 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
