| 产品编号 | bs-8475R |
| 英文名称 | FBXL15 Rabbit pAb |
| 中文名称 | FBXL15蛋白抗体 |
| 别 名 | FBXO37; Fbl15; JET; PSD; 0710008C12Rik; FXL15_HUMAN; FBXL15; F-box only protein 37; FXL15_MOUSE; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FBXL15: 201-300/300 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FBXL15 probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune responses, signaling cascades and developmental events) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors. FBL15 (F-box and leucine-rich repeat protein 15), also known as FBXL15, FBXO37, PSD or JET, is a 296 amino acid protein that contains one F-box domain and functions as a component of the SCF complex, possibly playing a role in the ubiquitination and subsequent degradation of target proteins. Function: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SMURF1, thereby acting as a positive regulator of the BMP signaling pathway. Required for dorsal/ventral pattern formation and bone mass maintenance. Also mediates ubiquitination of SMURF2 and WWP2. Subunit: Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXL15) composed of CUL1, SKP1, RBX1 and FBXL15. Subcellular Location: Cytoplasm. Similarity: Belongs to the FBXL15 family. Contains 1 F-box domain. Contains 5 LRR (leucine-rich) repeats. SWISS: Q9H469 Gene ID: 79176 Database links: Entrez Gene: 79176 Human Entrez Gene: 68431 Mouse Omim: 610287 Human SwissProt: Q9H469 Human SwissProt: Q91W61 Mouse Unigene: 380081 Human Unigene: 19973 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
