| 产品编号 | bs-9515R |
| 英文名称 | CTH Rabbit pAb |
| 中文名称 | 胱硫醚γ裂解酶抗体 |
| 别 名 | CGL_HUMAN; CTH; Cystathionine gamma lyase; Cystathionine gamma-lyase; Cysteine desulfhydrase; gamma cystathionase; gamma-cystathionase; Homoserine deaminase; Homoserine dehydratase; MGC9471; CSE; Cystathionase. |
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Specific References (3) | bs-9515R has been referenced in 3 publications.
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[IF=5.81] Heng-Jing Hu. et al. Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress. Front Pharmacol. 2021 Dec 7;12:690371 WB ; Mouse,Rat. 222
111
[IF=2.53] Shiina, Takahiko, et al. "Inhibitory action of hydrogen sulfide on esophageal striated muscle motility in rats." European Journal of Pharmacology (2016). WB ; Rat. 222
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[IF=2.1] Heng-Jing Hu. et al. Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA2 in THP‑1 monocytes via the p38MAPK pathway. Mol Med Rep. 2021 May;23(5):1-10 WB ; Human. 222
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| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Cow, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CTH/CSE/Cystathionase: 51-150/405 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Function: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into –SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Subunit: Homotetramer. Interacts with CALM in a calcium-dependent manner. Subcellular Location: Cytoplasm Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Similarity: Belongs to the trans-sulfuration enzymes family. SWISS: P32929 Gene ID: 1491 Database links: Entrez Gene: 1491 Human Omim: 607657 Human SwissProt: P32929 Human Unigene: 19904 Human |
| 产品图片 |
Sample:Liver (Mouse) Lysate at 40 ug
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 48 kD
Sample:Esophagus (Mouse) Lysate at 40 ug
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45 kD
Sample:K562 Cell (Human) Lysate at 40 ug
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-CTH antibody (bs-9515R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
