产品编号 | bs-9204R |
英文名称 | IGSF21 Rabbit pAb |
中文名称 | 免疫球蛋白超家族成员21抗体 |
别 名 | FLJ41177; IGSF 21; Immunoglobin superfamily member 21; immunoglobulin superfamily member 21; MGC15730; RP11 121A23.1; IGS21_HUMAN. |
研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 49 kDa |
检测分子量 | |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human IGSF21: 81-180/467 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
IGSF21 is a 467 amino acid secreted protein that contains two Ig-like (immunoglobulin-like) domains and is a member of the immunoglobulin superfamily. Members of this family of proteins usually localize to the cell membrane, and may act as receptors in the immune response pathways. The gene encoding IGSF21 maps to human chromosome 1p36.13 and mouse chromosome 4 D3. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. A large number of disease-associated genes are associated with chromosome 1, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma. Subcellular Location: Secreted Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q96ID5 Gene ID: 84966 Database links: Entrez Gene: 84966 Human SwissProt: Q96ID5 Human Unigene: 212511 Human |
产品图片 |
Sample: Cerebellum (mouse) Lysate at 40 ug
Primary: Anti- IGSF21(bs-9204R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49/69 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGSF21) Polyclonal Antibody, Unconjugated (bs-9204R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |