| 产品编号 | bs-9228R |
| 英文名称 | RNF149 Rabbit pAb |
| 中文名称 | 环指蛋白149抗体 |
| 别 名 | DNAPTP2; 1600023E10Rik; Gm15832; Greul4; RN149_HUMAN; RNF149; DNA polymerase-transactivated protein 2; RING finger protein 149; RING-type E3 ubiquitin transferase RNF149; 2.3.2.27; RN149_MOUSE; Goliath-related E3 ubiquitin-protein ligase 4; |
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Specific References (1) | bs-9228R has been referenced in 1 publications.
111
[IF=6.409] Xiuxiu Jin. et al. Identification of age-associated proteins and functional alterations in human retinal pigment epithelium. GENOM PROTEOM BIOINF. 2022 Jun;: WB ; Human. 222
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | 52 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF149: 31-130/400 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF149 (RING finger protein 149), also known as DNAPTP2 (DNA polymerase-transactivated protein 2) or E3 ubiquitin-protein ligase RNF149, is a 400 amino acid single-pass membrane protein that contains one PA (protease associated) domain and a single RING-type zinc finger. The gene encoding RNF149 maps to human chromosome 2, which consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2 including Harlequin icthyosis, sitosterolemia and Alstr鰉 syndrome. Function: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation. Subcellular Location: Membrane. Similarity: Contains 1 PA (protease associated) domain. Contains 1 RING-type zinc finger. SWISS: Q8NC42 Gene ID: 284996 Database links: Entrez Gene: 284996 Human Entrez Gene: 67702 Mouse SwissProt: Q8NC42 Human SwissProt: Q3U2C5 Mouse Unigene: 142074 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-RNF149 (bs-9228R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 52 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
