| 产品编号 | bs-9252R |
| 英文名称 | RNF130 Rabbit pAb |
| 中文名称 | 环指蛋白130抗体 |
| 别 名 | G1RP; G1RZFP; GOLIATH; GP; 2510042A13Rik; GOLI_HUMAN; RNF130; Goliath homolog (H-Goliath); RING finger protein 130; RING-type E3 ubiquitin transferase RNF130; 2.3.2.27; GOLI_MOUSE; G1-related zinc finger protein; Goliath homolog; GOLI_RAT; Goliath homolog |
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Specific References (1) | bs-9252R has been referenced in 1 publications.
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[IF=4.493] Qin Wentao. et al. Systematic Construction and Validation of a Novel Ferroptosis-Related Gene Model for Predicting Prognosis in Cervical Cancer. J IMMUNOL RES. 2022;2022:2148215 IHC ; Human. 222
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF130: 1-100/419 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF130 (ring finger protein 130), also known as GP, G1RZFP (G1-related zinc finger protein) or GOLIATH, is a 419 amino acid single-pass type I membrane protein that shares similarity with a Drosophila zinc-finger protein found in mesoderm known as g1. RNF130 contains one PA (protease associated) domain and a single RING-type zinc finger. Implicated in the regulation of growth factor withdrawal-induced apoptosis of myeloid precursor cells, RNF130 is encoded by a gene located on human chromosome 5q35.3 and mouse chromosome 11 B1.3. Function: May have a role during the programmed cell death of hematopoietic cells. Acts as an E3 ubiquitin-protein ligase. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Cytoplasm. Tissue Specificity: Ubiquitously expressed. Highly expressed in leukocytes. Not expressed in erythroblasts. Post-translational modifications: In vivo measurements suggest this protein is glycosylated (PubMed:19159218). In contrast, in vitro experiments failed to detect glycosylation (PubMed:16549277). Similarity: Contains 1 PA (protease associated) domain. Contains 1 RING-type zinc finger. SWISS: Q86XS8 Gene ID: 55819 Database links: Entrez Gene: 55819 Human Entrez Gene: 59044 Mouse SwissProt: Q86XS8 Human SwissProt: Q8VEM1 Mouse Unigene: 484363 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
