| 产品编号 | bs-9268R |
| 英文名称 | fbpB Rabbit pAb |
| 中文名称 | 结核分枝杆菌Ag85B/fbpB抗体 |
| 别 名 | A85B_MYCTU; Diacylglycerol acyltransferase/mycolyltransferase Ag85B; Mycobacterium tuberculosis Ag85B; M.tuberculosis Ag85b; EC:2.3.1.122; EC:2.3.1.20; DGAT; 30 kDa extracellular protein; Acyl-CoA:diacylglycerol acyltransferase; Antigen 85 complex B(85B; Ag85B); Extracellular alpha-antigen; Fibronectin-binding protein B(Fbps B); MTCY180.32; Rv1886c; |
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Specific References (2) | bs-9268R has been referenced in 2 publications.
111
[IF=2.738] Liang Wang et al. Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis. Dis Markers. 2020 May 20;2020:2509454. IHC ; Human. 222
111
[IF=1.785] Xiaoqian Shang. et al. Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Exp Ther Med. 2021 Jan;21(1):1-1 IHC ; Human. 222
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| 研究领域 | 细胞生物 免疫学 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Ag85B: 231-325/325 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall. Function: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Subcellular Location: Secreted. Similarity: Belongs to the mycobacterial A85 antigen family. |
| 产品图片 |
Sample:
Lane 1: Recombinant M.tuberculosis fbpB protein
Primary: Anti-fbpB (bs-9268R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
