| 产品编号 | bs-9320R |
| 英文名称 | HAUS8 Rabbit pAb |
| 中文名称 | HAUS8蛋白抗体 |
| 别 名 | HAUS augmin like complex subunit 8; HAUS augmin-like complex subunit 8; Hec1 interacting and centrosome associated 1; HEC1/NDC80 interacting centrosome associated protein 1; HICE 1; HICE1; MGC20533; NY SAR 48; Sarcoma antigen NY SAR 48; NYS48; HAUS8_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HAUS8 protein: 166-410/410 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NYS48 belongs to the HAUS8 family. It is required for stabilizing spindle integrity and chromosomal integrity. Function: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Subunit: Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect. Subcellular Location: Cytoplasm, cytoskeleton, Microtubule. Similarity: Belongs to the HAUS8 family. SWISS: Q9BT25 Gene ID: 93323 Database links:
Entrez Gene: 93323 Human
Omim: 613434 Human
SwissProt: Q9BT25 Human
Unigene: 404088 Human
|
| 产品图片 |
Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Mouse Spleen tissue lysates
Primary: Anti- HAUS8 (bs-9320R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Sample:
Lane 1: Mouse Lymph Lysates
Lane 2: Mouse SpleenLysates
Lane 3: Human Jurkat cell Lysates
Lane 4: Human MOLT4 cell Lysates
Lane 5: Human K562 cell Lysates
Primary: Anti-HAUS8 (bs-9320R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kDa
Observed band size: 45kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
