| 产品编号 | bs-9710R |
| 英文名称 | NOP2 Rabbit pAb |
| 中文名称 | 增殖相关核仁抗原抗体 |
| 别 名 | Proliferation Associated Nucleolar Antigen: NOL 1; NUCLEOLAR ANTIGEN p120; NOP2_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 细胞周期蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 89 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOL1: 1-100/812 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proliferation-associated nucleolar antigen is expressed in mid G1 phase with peak level during the S phase and a rapid degradation during late mitosis. Its expression in breast carcinoma is correlated with patient prognosis. Function: May play a role in the regulation of the cell cycle andthe increased nucleolar activity that is associated with the cellproliferation. May act as ribosomal RNA methyltransferase. Subunit: Interaction with MCRS1. Subcellular Location: Nuclear Similarity: Belongs to the methyltransferase superfamily. RsmB/NOPfamily. SWISS: P46087 Gene ID: 4839 Database links: Entrez Gene: 4839 Human Entrez Gene: 110109 Mouse Omim: 164031 Human SwissProt: P46087 Human SwissProt: Q922K7 Mouse Unigene: 534334 Human Unigene: 29203 Mouse Unigene: 32804 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NOP2) Polyclonal Antibody, Unconjugated (bs-9710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
