| 产品编号 | bs-9762R |
| 英文名称 | FGF18 Rabbit pAb |
| 中文名称 | 成纤维细胞生长因子18抗体 |
| 别 名 | FGF 18; FGF18; FGF-18; Fibroblast Growth Factor 18; ZFGF5; FGF18_HUMAN. |
 |
Specific References (2) | bs-9762R has been referenced in 2 publications.
111
[IF=11.501] WB ;
111
[IF=6.384] Anthony Estienne. et al. Endothelial cell-derived fibroblast growth factor-18 regulates ovarian function in sheep. 2022 Mar 21 IHC ; Sheep. 222
|
| 研究领域 | 细胞生物 信号转导 细胞凋亡 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Cow, Rabbit, Sheep, Goat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FGF18: 110-207/207 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. Function: Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation. Subunit: Interacts with FGFR3 and FGFR4. Subcellular Location: Secreted Similarity: Belongs to the heparin-binding growth factors family. SWISS: O76093 Gene ID: 8817 Database links: Entrez Gene: 8817 Human Entrez Gene: 14172 Mouse Omim: 603726 Human SwissProt: O76093 Human SwissProt: O89101 Mouse Unigene: 87191 Human Unigene: 339812 Mouse Unigene: 44478 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF18) Polyclonal Antibody, Unconjugated (bs-9762R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
