| 产品编号 | bs-9865R |
| 英文名称 | MYLK3 Rabbit pAb |
| 中文名称 | 肌球蛋白轻链激酶3抗体 |
| 别 名 | caMLCK; Cardiac MyBP C associated Ca/CaM kinase; Cardiac-MyBP-C-associated Ca/CaM kinase; MLC kinase; MLCK; MLCK2; Mylk3; MYLK3_HUMAN; Myosin light chain kinase 3; Putative myosin light chain kinase 3. |
| 研究领域 | 心血管 信号转导 激酶和磷酸酶 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MYLK3: 701-800/817 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and Myosin light chain kinases (MYLKs, also designated MLCKs). The MYLK kinases phosphorylate Myosin regulatory light chains to catalyze Myosin interaction with Actin filaments, resulting in contractile activity. MYLK3 (Myosin light chain kinase 3) is a 795 amino acid cardiac-specific protein that contains one protein kinase domain and belongs to the protein kinase superfamily. Like other MYLK kinases, MYLK3 is thought to play a role in smooth muscle contraction, specifically using magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of Myosin light chain proteins. Additionally, MYLK3 may regulate sarcomere assembly in heart tissue, possibly mediating proper heart function. Function: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity). Subcellular Location: Cytoplasm. Tissue Specificity: May play a role in smooth muscle contraction. Post-translational modifications: Phosphorylated on serine residues (By similarity). Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain. SWISS: Q32MK0 Gene ID: 91807 Database links: Entrez Gene: 91807 Human Omim: 612147 Human SwissProt: Q32MK0 Human Unigene: 130465 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MYLK3 polyclonal antibody, unconjugated (bs-9865R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- MYLK3 (bs-9865R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
