| 产品编号 | bs-4905R |
| 英文名称 | SORBS2 Rabbit pAb |
| 中文名称 | 精氨酸结合蛋白2抗体 |
| 别 名 | ArgBP2; Arg binding protein 2; Arg/Abl interacting protein 2; Arg/Abl interacting protein; ARGBP2; PRO0618; SRBS2_HUMAN; sorbin and SH3 domain containing 2. |
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Specific References (2) | bs-4905R has been referenced in 2 publications.
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[IF=9.452] Tong,et al.HSF1, in association with MORC2, downregulates ArgBP2 via the PRC2 family in gastric cancer cells.(2018) Biochimica Et Biophysica Acta. 1864:1104-1114. WB ; Human. 222
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[IF=5.542] Han L et al. The RNA‐binding protein SORBS2 suppresses hepatocellular carcinoma tumorigenesis and metastasis by stabilizing RORA mRNA. Liver Int. 2019 Jul 31. IHC ; Human. 222
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| 研究领域 | 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 124 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SORBS2/ArgBP2: 401-500/664 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SORBS2 is an adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. It can form a complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. Function: Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder. Subunit: Interacts with ABL, CBL, DNM1, DNM2, FLOT1, MLLT4/afadin, PTK2B/PYK2, SAPAP, SPTAN1, SYNJ1, SYNJ2, VCL/vinculin, and WASF (By similarity). Interacts with ABL1/c-Abl, ABL2/v-Abl/Arg, ACTN, AKT1, CBL, PALLD and PAK1. Subcellular Location: Cytoplasm, perinuclear region. Note=Found at the Z-disk sarcomeres, stress fibers, dense bodies and focal adhesion. Tissue Specificity: Abundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. Post-translational modifications: Ubiquitinated by CBL. Similarity: Contains 3 SH3 domains. Contains 1 SoHo domain. SWISS: O94875 Gene ID: 8470 Database links: Entrez Gene: 8470 Human Omim: 616349 Human SwissProt: O94875 Human Unigene: 619806 Human Unigene: 655143 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SORBS2) Polyclonal Antibody, Unconjugated (bs-4905R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
