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RabbitAnti-CLEC 4E Rabbit pAb  antibody (bs-8541R)
~~~促销,代码KXJ230206~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-8541R
英文名称 CLEC 4E Rabbit pAb
中文名称 C型凝集素4家族E抗体
别    名 C type lectin domain family 4 member E; C type lectin superfamily member 9; C-type(calcium dependent carbohydrate recognition domain) lectin superfamily member 9; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; CLC4E_HUMAN; CLEC 4E; CLEC4E Clec4e; CLECSF9; Macrophage inducible C type lectin; Macrophage-inducible C-type lectin; MINCLE.  
Specific References  (6)     |     bs-8541R has been referenced in 6 publications.
111 [IF=11.864] Darja Andreev. et al. Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin. J Clin Invest. 2020 Sep;130(9):4811-4830  IF ;  Mouse.  222
111 [IF=5.72] He, Yue, et al. "Mincle/Syk Signaling Pathway Contributes to Neuroinflammation after Subarachnoid Hemorrhage in Rats." Stroke; a journal of cerebral circulation 46.8 (2015): 2277.  WB, IHC ;  Rat.  222
111 [IF=4.302] Wang Y et al. Microglial Mincle receptor in the PVN contributes to sympathetic hyperactivity in acute myocardial infarction rat.(2018) J Cell Mol Med. Oct 24.  IF,WB ;  Rat.  222
111 [IF=3.201] Yuki Sato. et al. Limited Role of Mincle in the Host Defense against Infection with Cryptococcus deneoformans. Infect Immun. 2020 Oct;88(11):e00400-20  IHC ;  Mouse.  222
111 [IF=2.234] Liu XY et al. Acupuncture through (DU20) to (GB7) mitigates neurological impairment after intracerebral hemorrhage. Neural Regen Res. 2018 Aug;13(8):1425-1432.  IHC-P&WB ;  Rat.  222
111 [IF=1.166] Yu GR et al. Mincle in the innate immune response of mice fungal keratitis.Int J Ophthalmol. 2018 Apr 18;11(4):539-547.  IF ;  Mouse.  222
研究领域 细胞生物  信号转导  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,  (predicted: Mouse, Rat, Cow, Horse, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test IF=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 25 kDa
检测分子量
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CLECSF9: 51-150/219 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.

Function:
C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.

Subunit:
Monomer. Homodimer.

Subcellular Location:
Membrane.

Similarity:
Contains 1 C-type lectin domain.

SWISS:
Q9ULY5

Gene ID:
26253

Database links:

Entrez Gene: 26253 Human

Omim: 609962 Human

SwissProt: Q9ULY5 Human

Unigene: 236516 Human



产品图片
Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-CLEC 4E antibody (bs-8541R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (Hela); Negative blank control (A431) Green line : Primary Antibody (Rabbit Anti-CLEC 4E antibody (bs-8541R) ) Orange line:Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488) Hela(Positive)and A431(Negative control)cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).
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