产品编号 | bs-11159R |
英文名称 | PCDHGC3 Rabbit pAb |
中文名称 | 原钙粘蛋白γC3抗体 |
别 名 | Cadherin like 2; PC 43; PC43; PCDH 2; PCDH gamma C3; PCDH2; PCDHGC 3; Protocadherin 2; Protocadherin 43; Protocadherin gamma C3; Protocadherin gamma subfamily C 3; Protocadherin43; PCDGK_HUMAN. |
研究领域 | 信号转导 细胞粘附分子 细胞骨架 细胞膜蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human, Mouse, Rat, Horse, ) |
产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 98 kDa |
检测分子量 | |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2: 251-350/934 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDH2 (protocadherin-2), also known as PCDHGC3 (protocadherin gamma subfamily C, 3) or PC43, is a 934 amino acid single-pass type I membrane protein that contains six cadherin domains and belongs to the protocadherin gamma family. Functioning as a calcium-dependent cell-adhesion protein, PCDH2 is thought to be involved in the establishment and maintenance of neuronal connections within the brain. Multiple isoforms of PCDH2 exist due to alternative splicing events. Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. Subcellular Location: Cell membrane. Single-pass type I membrane protein. Similarity: Contains 6 cadherin domains. SWISS: Q9UN70 Gene ID: 5098 Database links: Entrez Gene: 5098 Human Omim: 603627 Human SwissProt: Q9UN70 Human Unigene: 368160 Human |
产品图片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human 293T cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-PCDHGC3 (bs-11159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 115 kDa
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |