| 产品编号 | bs-11237R |
| 英文名称 | TCF12 Rabbit pAb |
| 中文名称 | 转录因子12抗体 |
| 别 名 | bHLHb20; Class B basic helix-loop-helix protein 20; DNA binding protein HTF4; DNA-binding protein HTF4; E box binding protein; E-box-binding protein; HEB; HTF4; HTF4_HUMAN; TCF-12; Tcf12; Transcription factor 12; Transcription factor HTF-4; Transcription factor HTF4. |
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Specific References (2) | bs-11237R has been referenced in 2 publications.
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[IF=5.546] Mengjin Gao. et al. circHIPK3 regulates proliferation and differentiation of myoblast through the miR‐7/TCF12 pathway. 2021 Mar 21 WB ; Mouse. 222
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[IF=3.65] Chen, Qing-biao, et al. "Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer." Tumor Biology 39.6 (2017): 1010428317703924. WB, IHC-P ; Human. 222
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| 研究领域 | 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Horse, Rabbit, Zebrafish, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 73 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TCF12: 51-150/682 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression. Function: Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. Subunit: Contains 1 basic helix-loop-helix (bHLH) domain. Subcellular Location: Nucleus. Tissue Specificity: Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: Q99081 Gene ID: 6938 Database links: Entrez Gene: 6938 Human Omim: 600480 Human SwissProt: Q99081 Human Unigene: 511504 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (TCF12) Polyclonal Antibody, Unconjugated (bs-11237R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
