| 产品编号 | bs-11248R |
| 英文名称 | GRAF Rabbit pAb |
| 中文名称 | Rho GTP酶激活蛋白26抗体 |
| 别 名 | arhgap 26; ARHGAP26; FLJ42530; GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase; GTPase regulator associated with focal adhesion kinase pp125(FAK); KIAA0621; oligophrenin 1 like protein; Oligophrenin-1-like protein; oligophrenin1like pr |
| 研究领域 | 肿瘤 心血管 神经生物学 信号转导 细胞骨架 细胞外基质 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 92 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRAF1: 1-100/814 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cellular signaling by G-proteins is down-regulated by GTPase-activating proteins (GAPs), which increase the rate of GTP hydroylsis. The GTPase regulator associated with focal adhesion kinase (GRAF) has GAP activity toward Rho A and Cdc42, but not Rac1. GRAF is ubiquitously expressed with high levels in heart and brain. Expression of GRAF causes clearing of stress fibers and formation of long actin based filopodial-like extensions. Fusion of MLL with GRAF, MLL/GRAF, is included in a rare genetic subgroup of acute myeloid leukemia (AML) cases. Function: GTPase-activating protein for RHOA and CDC42. Subunit: Interacts with NYAP1, NYAP2 and MYO16 (By similarity). Binds to the C-terminus of PTK2/FAK1. Subcellular Location: Cell junction; focal adhesion. Cytoplasm; cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures. DISEASE: Defects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient. Similarity: Contains 1 PH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain. SWISS: Q9UNA1 Gene ID: 23092 Database links: Entrez Gene: 23092 Human Entrez Gene: 71302 Mouse Omim: 605370 Human SwissProt: Q9UNA1 Human SwissProt: Q6ZQ82 Mouse Unigene: 654668 Human Unigene: 329396 Mouse Unigene: 214162 Rat |
| 产品图片 |
Sample:
Cerebrum(Rat) Lysate at 40 ug
Cerebrum(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
HepG2(Human) Cell Lysate at 30 ug
Kidney(Rat) Lysate at 40 ug
Kidney(Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
U937(Human) Cell Lysate at 30 ug
Cerebellum(Mouse) Lysate at 40 ug
Primary: Anti-GRAF (bs-11248R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 38 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
