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RabbitAnti-IRE1 Rabbit pAb  antibody (bs-8680R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
50ul/1180.00元
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100ul/1980.00元
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200ul/2800.00元
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产品编号 bs-8680R
英文名称 IRE1 Rabbit pAb
中文名称 内质网核信号转导蛋白a1抗体
别    名 IRE1a; Endoplasmic reticulum(ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring protein 1; Inositol-requiring protein 1; IRE-1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; Ire1alpha; IRE1P; MGC163277; Protein kinase/endoribonuclease; Serine/threonine protein kinase/endoribonuclease IRE1.  
Specific References  (21)     |     bs-8680R has been referenced in 21 publications.
111 [IF=12.153] Zhu, Zhenglin. et al. The natural product salicin alleviates osteoarthritis progression by binding to IRE1α and inhibiting endoplasmic reticulum stress through the IRE1α-IκBα-p65 signaling pathway. EXP MOL MED. 2022 Nov;:1-13  WB ;  Rat.  222
111 [IF=6.614] Yiwei Yao. et al. CARMA3 deficiency aggravates angiotensin II-induced abdominal aortic aneurysms development interacting between endoplasmic reticulum and mitochondria. CAN J CARDIOL. 2023 Apr;:  IF,WB ;  Mouse.  222
111 [IF=5.793] Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16  WB ;  Human.  222
111 [IF=5.075] Angelica D. Reyes-Perea. et al. The Mycotoxin De-Epoxy-Deoxynivalenol (DOM-1) Increases Endoplasmic Reticulum Stress in Ovarian Theca Cells. TOXINS. 2023 Mar;15(3):228  WB ;  Bovine.  222
111 [IF=4.868] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742.  WB ;  Mouse.  222
111 [IF=4.86] Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226.  WB ;  Human.  222
111 [IF=4.294] Mei Zhang. et al. Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Neurotoxicology. 2022 Jan;88:187  WB ;  Human.  222
111 [IF=4.19] Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015).  WB ;  Human.  222
111 [IF=4.07] He, Wencheng, et al. "CSFV Infection Up-Regulates the Unfolded Protein Response to Promote Its Replication." Frontiers in Microbiology 8 (2017): 2129.  WB ;  Pig.  222
111 [IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.  WB ;  Human.  222
111 [IF=3.457] Lu, Y. et al. Protective effects of Danzhi jiangtang capsule on vascular endothelial damages induced by high-fat diet and palmitic acid. (2018) Biomedicine & Pharmacotherapy, 107, 1631–1640.  WB ;  Rat.  222
111 [IF=3.29] Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176.  WB ;  Human.  222
111 [IF=3.28] Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13.  WB ;  Mouse.  222
111 [IF=3.12] Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).  WB ;  Mouse.  222
111 [IF=3.038] Jiajia Chenet al. Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. Neurochem Res . 2020 Oct;45(10):2302-2311.  WB, IHC ;  rat.  222
111 [IF=2.791] Yang S et al. Induction of the unfolded protein response (UPR) during pseudorabies virus infection. Vet Microbiol. 2019 Dec;239:108485.  WB ;  Pig.  222
111 [IF=2.776] He Q et al. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ Toxicol Pharmacol. 2018 Oct;63:6-15.  WB ;  Mouse.  222
111 [IF=2.638] Yu-Dong Wei. et al. Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zool Res. 2021 Jan 18; 42(1): 14–27  WB ;  Goat.  222
111 [IF=2.147] Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951  IF ;  Human.  222
111 [IF=2.092] Zhang Hao. et al. Protective effects of pterostilbene against hepatic damage, redox imbalance, mitochondrial dysfunction, and endoplasmic reticulum stress in weanling piglets. J Anim Sci. 2020 Oct;98(10):  WB ;  Pig.  222
111 [IF=1.664] Kishi,et al.Pro-metastatic signaling of the trans fatty acid elaidic acid is associated with lipid rafts.(2018) Oncology Letters. 15:4423-4426.  WB ;  Mouse.  222
研究领域 细胞生物  信号转导  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 105 kDa
检测分子量
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IRE1a: 252-260/977 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Subunit:
Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.

Subcellular Location:
Endoplasmic reticulum membrane.

Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.

SWISS:
O75460

Gene ID:
10595

Database links:

Entrez Gene: 2081 Human

Entrez Gene: 78943 Mouse

Entrez Gene: 498013 Rat

Omim: 604033 Human

SwissProt: O75460 Human

SwissProt: Q9EQY0 Mouse

Unigene: 133982 Human

Unigene: 592041 Human

Unigene: 700027 Human

Unigene: 20452 Mouse

Unigene: 340943 Mouse

Unigene: 226435 Rat



产品图片
Sample: Lane 1: Human Raji cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-IRE1 (bs-8680R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kDa Observed band size: 135 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (bs-8680R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Raji. Primary Antibody (green line): Rabbit Anti-IRE1 antibody (bs-8680R ) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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