| 产品编号 | bs-11325R |
| 英文名称 | Nova2 Rabbit pAb |
| 中文名称 | 神经肿瘤Nova2抗原抗体 |
| 别 名 | ANOVA; Astrocytic NOVA1 like RNA binding protein; Gm1424; NOVA2_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 细胞类型标志物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Chicken, Dog, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nova2: 401-492/492 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nova-1 and Nova-2 are members of a superfamily of protein regulators of RNA metabolism in neurons. Both are nuclear RNA binding proteins with K homology motifs, conserved protein sequences which bind to RNA (1,2). Nova proteins, normally sequestered in the central nervous system, are expressed by systemic tumors in patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA) (3,4). Nova-1 is expressed in the hindbrain and ventral spinal cord and Nova-2 is expressed in the neocortex and hippocampus (4). Nova-1 is necessary for regulating neuron-specific alternative splicing of the glycine receptor Alpha2 pre-mRNA (5). Function: NOVA2 may regulate RNA splicing or metabolism in a specific subset of developing neurons. It binds single strand RNA. Subunit: Interacts with PTBP2; the interaction is direct (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Brain. Expression restricted to astrocytes. Similarity: Contains 3 KH domains. SWISS: Q9UNW9 Gene ID: 4858 Database links: Entrez Gene: 4858 Human Entrez Gene: 384569 Mouse Omim: 601991 Human SwissProt: Q9UNW9 Human Unigene: 375439 Human Unigene: 262724 Mouse |
| 产品图片 |
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Nova2 antibody(bs-11325R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
