| 产品编号 | bs-11379R |
| 英文名称 | Syntenin 1 Rabbit pAb |
| 中文名称 | 多配体聚糖结合蛋白1抗体 |
| 别 名 | Sdcbp; MDA-9; MDA9; Melanoma differentiation-associated protein 9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; Scaffold protein Pbp1; SDCB1_HUMAN; Sdcbp; ST1; SYCL; Syndecan binding protein 1; Syndecan-binding protein 1; Syntenin 1; Syntenin-1; TACIP18. |
| 研究领域 | 细胞生物 神经生物学 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Syntenin 1: 121-220/298 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Syntenin-1 (also known as syntenin, syndecan binding protein, melanoma differentiation-associated protein 9 or proTGF-alpha cytoplasmic domain-interacting protein 18) is a protein that binds to the cytoplasmic domains of the syndecans in yeast 2-hybrid screens and other assays. Syntenin-1 contains a tandem repeat of PDZ domains that reacts with the FYA (phe-tyr-ala) carboxy-terminal amino acid sequence of the syndecans. It may function as an adaptor that couples syndecans to cytoskeletal proteins or cytosolic downstream signal-effectors. Syntenin-1 colocalizes and interacts specifically with immature, intracellular forms of proTGF-alpha. It is a human gamma interferon responsive protein. Syntenin-1 contains PSD-95/Discs large/zO-1 (PDZ) domains and associates with the cytoplasmic tail of the IL-5Ralpha. It directly associates with the transcription factor Sox-4. The PDZ proteins PICK1, GRIP, ABP and syntenin-1 bind multiple glutamate receptor subtypes. Function: Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway. Subunit: Monomer and homodimer. Interacts with SDC1, SDC2, SDC3, SDC4, NRXN2, EPHA7, EPHB1, NF2 isoform 1, TGFA and IL5RA. Interacts with neurofascin, SDCBP2 and PTPRJ. Subcellular Location: Cell junction, focal adhesion. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus. Melanosome. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Note=Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta. Post-translational modifications: Phosphorylated on tyrosine residues. Similarity: Contains 2 PDZ (DHR) domains. SWISS: O00560 Gene ID: 6386 Database links: Entrez Gene: 6386 Human Entrez Gene: 53378 Mouse Omim: 602217 Human SwissProt: O00560 Human SwissProt: O08992 Mouse Unigene: 200804 Human Unigene: 247473 Mouse Unigene: 483278 Mouse Unigene: 4309 Rat |
| 产品图片 |
Tissue/cell: Mouse Embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Syntenin 1 Polyclonal Antibody, Unconjugated(bs-11379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
