| 产品编号 | bs-11443R |
| 英文名称 | NMS Rabbit pAb |
| 中文名称 | 神经调节肽S抗体 |
| 别 名 | Neuromedin S; Neuromedin-S; NeuromedinS; Nms; NMS_HUMAN; Prepro NMS. |
| 研究领域 | 肿瘤 心血管 神经生物学 信号转导 生长因子和激素 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 4 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neuromedin S: 81-153/153 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NMS is a 153 amino acid secreted protein that belongs to the NmU family. NMS is implicated in the regulation of circadian rhythms through autocrine and/or paracrine actions. The gene that encodes NMS consists of approximately 12,799 bases and maps to human chromosome 2q11.2. Consisting of 237 million bases and encoding over 1,400 genes, chromosome 2 makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. Function: NMS (neuromedin S) is a 36-amino acid neuropeptide specifically expressed in the suprachiasmatic nucleus (SCN) of the hypothalamus. NMS shares a C-terminal core structure with NMU. NMS mRNA is highly expressed in the central nervous system, spleen and testis. NMS may be implicated in the regulation of circadian rhythms and feeding behavior. Subcellular Location: Secreted. Similarity: Belongs to the NmU family. SWISS: Q5H8A3 Gene ID: 129521 Database links: Entrez Gene: 129521 Human SwissProt: Q5H8A3 Human Unigene: 567676 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Primary: Anti-NMS (bs-11443R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 4 kDa
Observed band size: 20 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NMS) Polyclonal Antibody, Unconjugated (bs-11443R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NMS) Polyclonal Antibody, Unconjugated (bs-11443R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
