| 产品编号 | bs-11517R |
| 英文名称 | FDFT1 Rabbit pAb |
| 中文名称 | 法尼基二磷酸法尼基转移酶1/鲨烯合成酶抗体 |
| 别 名 | DGPT; ERG9; Farnesyl diphosphate farnesyltransferase 1; Farnesyl diphosphate farnesyltransferase; Farnesyl-diphosphate farnesyltransferase; FDFT_HUMAN; FDFT1; FPP:FPP farnesyltransferase; Presqualene di diphosphate synthase; SQS; Squalene synthase; Squalene synthetase; SS. |
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Specific References (1) | bs-11517R has been referenced in 1 publications.
111
[IF=2.98] Facchini, Gustavo, et al. "Toxic effects of phytol and retinol on human glioblastoma cells are associated with modulation of cholesterol and fatty acid biosynthetic pathways." Journal of Neuro-Oncology (2017): 1-9. WB ; Human. 222
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| 研究领域 | 肿瘤 细胞生物 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FDFT1: 321-417/417 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Several proteins mediate the biosynthesis of cholesterol. The first specific step in the cholesterol biosynthetic pathway is the conversion of transfarnesyl-diphosphate to Squalene, which is catalyzed by the endoplasmic reticulum membrane-associated enzyme Squalene synthetase, also designated Squalene synthase and Farnesyl-diphosphate farnesyltransferase. Squalene synthetase is located at a branch point in the mevalonate pathway and is also involved in isoprenoid biosynthesis. Squalene epoxidase, also designated Squalene monooxygenase, is a multi-pass microsomal membrane-associated enzyme that catalyzes the first oxygenation step in sterol biosynthesis and most likely functions as one of the rate-limiting enzymes in this pathway. Squalene epoxidase may form a complex with Squalene synthetase. Function: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3. Subunit: Monomer Subcellular Location: Endoplasmic reticulum membrane. Similarity: Belongs to the phytoene/squalene synthase family. SWISS: P37268 Gene ID: 2222 Database links: Entrez Gene: 2222 Human Entrez Gene: 14137 Mouse Omim: 184420 Human SwissProt: P37268 Human SwissProt: P53798 Mouse Unigene: 593928 Human Unigene: 474432 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-FDFT1 (bs-11517R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
