| 产品编号 | bs-11913R |
| 英文名称 | NPDC1 Rabbit pAb |
| 中文名称 | 神经细胞增殖和分化调控蛋白1抗体 |
| 别 名 | AI314472; CAB1; Neural proliferation differentiation and control protein 1; NPDC-1; NPDC1; NPDC1_HUMAN. |
| 研究领域 | 神经生物学 细胞凋亡 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPDC1: 75-180/325 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NPDC-1 (Neural Proliferation Differentiation and Control-1) is expressed in neurons once they have stopped dividing and begun to differentiate. NPDC-1 is transported from the Golgi apparatus via vesicles before becoming internalized by endosomes at the cell membrane. NPDC-1 interacts with Cdk2, D-type cyclins, and the transcription factor E2F1. This interaction can lead to an increased replication time, and might have implications in final neural differentiation and apoptosis. NPDC-1 has been shown to colocalize with synaptic vesicle proteins: synaptophysin, synaptobrevin 2, and Rab3 GEP (Rab3 GTP/GDP exchange protein). One function of NPDC-1 is to regulate retinoic acid-mediated events by directly interacting with retinoid receptors. The amino acid sequence of NPDC-1 is highly conserved between mouse, rat, and human. Function: Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation. Subcellular Location: Membrane; Single-pass membrane protein (Potential). Tissue Specificity: Strongly expressed in adult brain; especially in hippocampus, frontal lobe and temporal lobe. Similarity: Belongs to the NPDC1/cab-1 family. SWISS: Q9NQX5 Gene ID: 56654 Database links: Entrez Gene: 56654 Human Entrez Gene: 18146 Mouse Omim: 605798 Human SwissProt: Q9NQX5 Human SwissProt: Q64322 Mouse Unigene: 719906 Human Unigene: 1131 Mouse Unigene: 5802 Rat |
| 产品图片 |
Sample: Uterus (mouse) Lysate at 40 ug
Primary: Anti- NPDC1(bs-11913R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample: Large intestine (mouse) Lysate at 40 ug
Primary: Anti- NPDC1(bs-11913R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
