| 产品编号 | bs-11971R |
| 英文名称 | RAMP2 Rabbit pAb |
| 中文名称 | 受体活性修饰蛋白2抗体 |
| 别 名 | Calcitonin receptor like receptor activity modifying protein 2; CRLR activity modifying protein 2; OTTMUSP00000002615; OTTMUSP00000037624; Receptor(calcitonin) activity modifying protein 2; Receptor activity modifying protein 2; Receptor activity modifying protein 2 isoform; RP23-281C18.6; RAMP2_HUMAN. |
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Specific References (1) | bs-11971R has been referenced in 1 publications.
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[IF=1.86] Qiao, Xi, et al. "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction."Nephrology (2015). WB ; Rat. 222
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| 研究领域 | 肿瘤 细胞生物 信号转导 生长因子和激素 细胞膜受体 G蛋白偶联受体 G蛋白信号 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RAMP2: 81-175/175 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. Function: RAMP2 (Receptor activity-modifying protein 2) transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. RAMP2 acts as a receptor for adrenomedullin (AM) together with CALCRL. Co-expression of Receptor activity-modifying protein 2 (RAMP2) with the calcitonin-receptor-like-receptor (CRLR) results in the production of a functional adrenomedullin (ADM) receptor. RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor. RAMP2 is a heterodimer of CALCRL and RAMP2. RAMP2 is strongly expressed in lung, breast, immune system and fetal tissues. RAMP2 belongs to the RAMP family. Subunit: Heterodimer of CALCRL and RAMP2 Subcellular Location: Cell Membrane; Single-pass type I membrane protein Tissue Specificity: Strongly expressed in lung, breast, immune system and fetal tissues. Similarity: Belongs to the RAMP family. SWISS: O60895 Gene ID: 10266 Database links: Entrez Gene: 10266 Human Omim: 605154 Human SwissProt: O60895 Human Unigene: 514193 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with RAMP2 polyclonal antibody, unconjugated (bs-11971R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
