| 产品编号 | bs-12067R |
| 英文名称 | TLX2 Rabbit pAb |
| 中文名称 | T淋巴细胞白血病同源蛋白2抗体 |
| 别 名 | Enx; homeo box 11 like 1; Homeobox protein Hox 11L1; Homeobox TLX 2; HOX11L1; NCX; Neural crest homeobox protein; PMUR10F; T cell leukemia homeobox protein 2; T cell leukemia, homeobox 2. |
| 研究领域 | 肿瘤 神经生物学 细胞分化 淋巴细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TLX2: 101-200/284 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
T-cell leukemia homeobox protein 2 (TLX2), also known as homeobox protein Hox-11L1 (HOX11L1), neural crest homeobox protein (NCX) or ENX, is a 284 amino acid member of the TLX homeobox family. The mouse homolog, Tlx2, has been detected in dorsal-root ganglia, cranial and entric-nerve gangla, parasympathetic ganglia and adrenal glands in mouse embryos and in the adrenal glands, intestine and heart of adult mice. The expression pattern of Tlx2, which is restricted to tissues derived from nerual crest cells, suggests that it may play a role in the proliferation or differentiation of the enteric peripheral nervous system. TLX2, which is localized to the nucleus, is highly homologous to mouse Tlx2, and shares several critical domains, including an enhancer element in the promotor that is crucial for tissue-specific expression. Mutations in the gene encoding mouse Tlx2 lead to congenital anomalies closely resembling neuronal intestinal dysplasia in humans. Thus, TLX2 is thought to play a role in this disease, which is a rare condition characterized by hyperplasia of submucosal plexus with giant submucosal ganglia and increased acetylcholinesterase activity in nerve fiber around submucosal blood vessels. Function: TLX2 contains 1 homeobox DNA-binding domain and the function remains unknown. In the mouse, this gene is expressed from embryonic day 9.5 through day 13.5 and is detectable in the dorsal root ganglia, cranial and enteric nerve ganglia and adrenal glands. In adult animals its expression is limited to the adrenal gland and the intestine. TLX2 may be involved in the proliferation and/or differentiation of enteric peripheral nervous system. Subcellular Location: Nuclear Similarity: Contains 1 homeobox DNA-binding domain. SWISS: O43763 Gene ID: 3196 Database links: Entrez Gene: 3196 Human Entrez Gene: 21909 Mouse Omim: 604240 Human SwissProt: O43763 Human SwissProt: Q61663 Mouse |
| 产品图片 |
Sample:HepG2 (Human)CellLysate at 40 ug
Primary: Anti-TLX2(bs-12067R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 30kD
Observed band size: 30kD
Sample:Cerebrum (Rat)Lysate at 40 ug
Primary: Anti-TLX2(bs-12067R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 30kD
Observed band size: 30kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
