| 产品编号 | bs-12153R |
| 英文名称 | TXLNB Rabbit pAb |
| 中文名称 | β-taxilin蛋白抗体 |
| 别 名 | beta-taxilin; C6orf198; dJ522B19.2; DKFZp451A175; hMDP77; LST001; MDP77; Muscle-derived protein 77; RP3-522B19.2; Txlnb; TXLNB_HUMAN. |
| 研究领域 | 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 76 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TXLNB: 331-430/684 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Beta-taxilin promotes nerve regeneration and may be involved in intracellular vesicle transport. Expressed predominantly in cardiac and skeletal muscle, beta-taxilin binds to the coiled coil region of the syntaxin family members STX1A, STX3A, and STX4A. Beta- and gamma-taxilins, bind to the alpha subunit of the nascent polypeptide-associated complex (NAC) and affect its nuclear distribution, suggesting that the taxilin family is involved not only in the translational process through its interaction with NAC but also in the
transcriptional process through its interaction with alphaNAC alone. Function: Promotes motor nerve regeneration (By similarity). May be involved in intracellular vesicle traffic. Subunit: Binds to the C-terminal coiled coil region of syntaxin family members STX1A, STX3A and STX4A. Has a preference for STX1A. Tissue Specificity: Expressed in skeletal muscle. Similarity: Belongs to the taxilin family. SWISS: Q8N3L3 Gene ID: 167838 Database links: Entrez Gene: 167838 Human Entrez Gene: 378431 Mouse Omim: 611438 Human SwissProt: Q8N3L3 Human SwissProt: Q8VBT1 Mouse Unigene: 535820 Human Unigene: 283493 Mouse Unigene: 165855 Rat |
| 产品图片 |
Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-TXLNB (bs-12153R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TXLNB) Polyclonal Antibody, Unconjugated (bs-12153R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
