| 产品编号 | bs-7157R |
| 英文名称 | Mitoferrin 2/SLC25A28 Rabbit pAb |
| 中文名称 | 线粒体铁转运蛋白2抗体 |
| 别 名 | MFRN2; Mitochondrial iron transporter 2; Mitochondrial RNA splicing protein 3/4; Mitoferrin 2; Mitoferrin2; Mitoferrin-2; MRS3/4; MRS4L; NPD016; Putative mitochondrial solute carrier; MFRN2_HUMAN; SLC25A28. |
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Specific References (3) | bs-7157R has been referenced in 3 publications.
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[IF=6.312] Christine Fischer. et al. Dietary Iron Overload and Hfe−/− Related Hemochromatosis Alter Hepatic Mitochondrial Function. Antioxidants-Basel. 2021 Nov;10(11):1818 WB ; Mouse. 222
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[IF=6.117] Jingjing Liu. et al. Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction. J NUTR BIOCHEM. 2022 Dec;:109258 WB ; Mouse, Human. 222
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[IF=3.383] Dongchen Wang. et al. Mitoferrin 2 deficiency prevents mitochondrial iron overload-induced endothelial injury and alleviates atherosclerosis. Exp Cell Res. 2021 May;402:112552 IF ; Mouse. 222
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| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 新陈代谢 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mitoferrin 2: 271-364/364 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mitoferrin 2, also known as MRS3/4 (mitochondrial RNA-splicing protein 3/4 homolog), mitochondrial iron transporter 2, NPD016, MRS4L or SLC25A28 (solute carrier family 25 member 28), is a 364 amino acid multi-pass membrane protein of the mitochondrial inner membrane that mediates iron uptake. Mitoferrin 2 is thought to play a role in heme synthesis of hemoproteins and iron-sulfur cluster assembly. Ubiquitously expressed, Mitoferrin 2 is found at high levels in skeletal muscle, heart, placenta, kidney, lung, liver, brain and pancreas. Mitoferrin 2 is a member of the mitochondrial carrier family and undergoes alternative splicing events to produce four isoforms. Mitoferrin 2 contains three solcar repeats and is encoded by a gene that maps to human chromosome 10q24.2. Function: SLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. Subcellular Location: Mitochondrion inner membrane; Multi pass membrane protein. Note: Isoform 1 and isoform 2 are both localized in the mitochondrion. Tissue Specificity: Ubiquitous. Expressed in placenta, lung, kidney, pancreas, liver, brain, skeletal muscle and heart. Similarity: Belongs to the mitochondrial carrier (TC 2.A.29) family. Contains 3 Solcar repeats. SWISS: Q96A46 Gene ID: 81894 Database links: Entrez Gene: 81894 Human Entrez Gene: 246696 Mouse Omim: 609767 Human SwissProt: Q96A46 Human SwissProt: Q8R0Z5 Mouse |
| 产品图片 |
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Primary:
Anti-Mitoferrin 2/SLC25A28 (bs-7157R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 37 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
