| 产品编号 | bs-12330R |
| 英文名称 | Trim22 Rabbit pAb |
| 中文名称 | 环指蛋白94抗体 |
| 别 名 | 50 kDa stimulated trans acting factor; RING finger protein 94; RNF94; Staf-50; RNF94; Staf 50; STAF50; Stimulated trans acting factor(50 kDa); Stimulated Trans Acting Factor(homolog of Mouse Rpt 1 gene); Trim22; Tripartite binding motif 22; Tripartite motif containing protein 22; TTRIM22RIM22; TRI22_HUMAN. |
| 研究领域 | 细胞生物 免疫学 干细胞 淋巴细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human Trim22/Staf-50/RNF94: 231-330/498 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. Staf-50 (50 kDa-stimulated trans-acting factor), also known as TRIM22 (tripartite motif-containing 22), RNF94 or GPSTAF50, is a 498 amino acid cytoplasmic protein that belongs to the TRIM family and, characteristic of TRIM family members, contains one RING-type zinc finger, one B box-type zinc finger and one SPRY domain. Induced by IFN-å and IFN-∫, Staf-50 is strongly expressed in ovary, spleen, thymus and peripheral blood leukocytes where it is thought to mediate the antiviral effects of IFN proteins. Additionally, Staf-50 is present in leukemic cells, suggesting a role in cancer formation and metastasis. Staf-50 exists as two alternatively spliced isoforms which are encoded by a gene that maps to human chromosome 11. Function: Trim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms. Subunit: Interacts with HIV-1 Gag polyprotein; this interaction seems to reduce gag production or virus budding. Interacts with EMCV protease 3C; this interaction leads to viral protease ubiquitination. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues. Post-translational modifications: Auto-ubiquitinated. Similarity: Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: Q8IYM9 Gene ID: 10346 Database links: Entrez Gene: 10346 Human Omim: 606559 Human SwissProt: Q8IYM9 Human Unigene: 501778 Human |
| 产品图片 |
Sample:
Raji Cell (Human) Lysate at 40 ug
U937 Cell (Human) Lysate at 40 ug
Primary: Anti-Trim22 (bs-12330R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
