| 产品编号 | bs-12387R |
| 英文名称 | KIF13B Rabbit pAb |
| 中文名称 | 驱动蛋白家族蛋白13B抗体 |
| 别 名 | GAKIN; Guanylate kinase associated kinesin; KI13B_HUMAN; KIAA0639; KIF 13B; KIF13B; Kinesin 13B; Kinesin family member 13B; Kinesin like protein GAKIN; Kinesin like protein KIF13B; Kinesin-like protein GAKIN; Kinesin-like protein KIF13B; OTTHUMP0000022564 |
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Specific References (1) | bs-12387R has been referenced in 1 publications.
111
[IF=8.713] Oscar Marcelo Lazo. et al. Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport. ELIFE. 2023 Mar
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| 研究领域 | 细胞生物 神经生物学 信号转导 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 203 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIF13B: 351-450/1826 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
KIF13B is also known as Kinesin-like protein GAKIN or GAKIN and is a 1,826 amino acid protein that is widely expressed in tissues, with highest expression in brain and kidney. KIF13B is localized to the cytoplasm, as well as to the cytoskeleton, and is thought to be a microtubule-dependent motor protein which is able to bind to a variety of proteins in order to traffic them to various locations throughout the cell. KIF13B belongs to the kinesin-like protein family and possesses three domains typical of the kinesin-like protein family, namely an N-terminal motor domain with an ATP-binding motif, an FHA domain which is known to bind diverse cargos and a large stalk domain involved in protein-protein binding. Additionally, KIF13B has a microtubule-interacting sequence which is known as the CAP-Gly domain at its C-terminus. The CAP-Gly domain is highly conserved domain among eukaryotes, and in humans, defects in the CAP-Gly domain are implicated in many diseases affecting the trafficking of vesicles, neuromuscular junctions and lysosome proliferation. Function: May be involved in reorganization of the cortical cytoskeleton. May be functionally important for the intracellular trafficking of MAGUKs and associated protein complexes. Subunit: Binds to DLG1 and DLG4. Interacts (when phosphorylated at Ser-1381 and Ser-1410) with 14-3-3. Subcellular Location: Cytoplasm; cytoskeleton. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the kinesin-like protein family. Contains 1 CAP-Gly domain. Contains 1 FHA domain. Contains 1 kinesin-motor domain. SWISS: Q9NQT8 Gene ID: 23303 Database links: Entrez Gene: 23303 Human Entrez Gene: 16554 Mouse Omim: 607350 Human SwissProt: Q9NQT8 Human Unigene: 444767 Human |
| 产品图片 |
Sample:
Ovary (Rat) Lysate at 40 ug
Primary: Anti-KIF13B (bs-12387R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 203 kD
Observed band size: 203 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIF13B) Polyclonal Antibody, Unconjugated (bs-12387R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIF13B) Polyclonal Antibody, Unconjugated (bs-12387R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
