| 产品编号 | bs-12418R |
| 英文名称 | BAMBI Rabbit pAb |
| 中文名称 | 肿瘤转移抑制蛋白BAMBI抗体 |
| 别 名 | BAMBI; BAMBI_HUMAN; BMP and activin membrane bound inhibitor homolog; BMP and activin membrane-bound inhibitor homolog; NMA; Non metastatic gene A protein; Non-metastatic gene A protein; Putative transmembrane protein NMA. |
 |
Specific References (5) | bs-12418R has been referenced in 5 publications.
111
[IF=17.521] Hongwei Chen. et al. Dissecting Heterogeneity Reveals a Unique BAMBIhighMFGE8high Subpopulation of Human UC-MSCs. Advanced Science. 2022 Nov;:2202510 WB ; Human. 222
111
[IF=5.23] He, Yao, et al. "LPS/TLR4 Signaling Enhances TGF-β Response Through Downregulating BAMBI During Prostatic Hyperplasia." Scientific reports 6 (2016): 27051. IHC-P, IF(ICC) ; Human. 222
111
[IF=2.812] Yaqi Zhang. et al. Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep. MOL REPROD DEV. 2023 Feb;: IF,WB ; Sheep. 222
111
[IF=2.08] Bai, Long, et al. "Identification and Expression Analyses of BAMBI Mediated by FSH in Swine Luteinizing Granulosa Cells." Theriogenology (2014). Pig. 222
111
[IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166. WB ; Mouse. 222
|
| 研究领域 | 肿瘤 干细胞 生长因子和激素 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BAMBI: 101-200/260 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines. Function: Negatively regulates TGF-beta signaling. Subcellular Location: Membrane. Tissue Specificity: High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. Similarity: Belongs to the BAMBI family. SWISS: Q13145 Gene ID: 25805 Database links: Entrez Gene: 25805 Human Entrez Gene: 68010 Mouse Omim: 604444 Human SwissProt: Q13145 Human SwissProt: Q9D0L6 Mouse Unigene: 533336 Human Unigene: 284863 Mouse Unigene: 25267 Rat |
| 产品图片 |
Sample:
RAW264.7 (Mouse) CellLysate at 30 ug
Primary: Anti-BAMBI (bs-12418R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Primary:
Anti-BAMBI (bs-12418R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 30 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-BAMBI (bs-12418R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated(bs-12418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
