| 产品编号 | bs-15164R |
| 英文名称 | C3IP1 Rabbit pAb |
| 中文名称 | 补体C3IP1抗体 |
| 别 名 | C3IP1; DKIR; XELAEV_18012174mg; klhl12; wu:fi60g03; zgc:92570; KLH12_BOVIN; KLH12_HUMAN; CUL3-interacting protein 1; DKIR homolog (hDKIR); KLH12_MOUSE; KLH12_RAT; KLH12_XENLA; KLH12_DANRE; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63 kDa |
| 检测分子量 | 65 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C3IP1 : 1-100/568 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
KLHL12 is a 568 amino acid protein that is a component of an ubiquitin-protein E3 ligase complex, which includes at least CUL-3. KLHL12 is a substrate-specific adapter for the complex, which negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of Dvl-3. KLHL12 contains six Kelch repeats and one BTB (POZ) domain, which is required for interaction with CUL-3. KLHL12 has highest expression in testis, with lower levels found in the submandibular salivary gland. The gene that encodes KLHL12 maps to human chromosome 1q32.1 Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. Subunit: Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A. Subcellular Location: Cytoplasmic vesicle, COPII-coated vesicle. Tissue Specificity: Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland. Similarity: Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. SWISS: Q53G59 Gene ID: 59349 Database links: Entrez Gene: 59349 Human Entrez Gene: 240756 Mouse Entrez Gene: 431860 Xenopus laevis Entrez Gene: 492362 Zebrafish Omim: 614522 Human SwissProt: Q53G59 Human SwissProt: Q8BZM0 Mouse SwissProt: Q6NRH0 Xenopus laevis SwissProt: Q5U374 Zebrafish Unigene: 706793 Human Unigene: 478061 Mouse Unigene: 87398 Mouse Unigene: 15513 Rat Unigene: 32296 Xenopus laevis Unigene: 81655 Zebrafish |
| 产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Bone marrow (Mouse) Lysate at 40 ug
Primary: Anti-C3IP1 (bs-15164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 65 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
