| 产品编号 | bs-13262R |
| 英文名称 | GABPB2 Rabbit pAb |
| 中文名称 | GA结合蛋白转录因子β/GABP-β1/GABP-β2抗体 |
| 别 名 | BABPB2; E4TF1; E4TF1-47; E4TF1-53; E4TF1B; GABPB; GABPB-1; GABPB2; NRF2B1; NRF2B2; GABPB-2; GABPB1-1; GABPB1-2; Gabpb1; Nrf-2beta; Nrf2b; GABP1_HUMAN; GABP subunit beta-1; GABP subunit beta-2 (GABPB-2); Nuclear respiratory factor 2; Transcription factor E |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABPB2/GABPB1: 301-395/395 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The transcription factor GA-binding protein (GABP) is composed of two subunits, the Ets-related GABP-alpha and a GABP-alpha-associated subunit, GABP beta. GABP alpha binds to a specific DNA sequence and GABP beta exists as b1 and b2 splice variants that differ in their C-termini. In primary neuronal cultures, GABP beta is expressed in both the cytoplasm and the nucleus, whereas GABP alpha is expressed mainly in the nucleus. GABP is constitutively expressed as either a GABP alpha beta heterodimer or a GABP alpha b heterotetramer, both of which can modify GABP-dependent transcription in vitro and in vivo . The GABP alpha beta tetrameric complex performs many different functions, such as stimulating transcription of the adenovirus E4 gene, differentially activating BRCA1 expression in human breast cell lines, potentiating Tat-mediated activation of long terminal repeat promoter transcription and viral replication in certain cell types, acting as a coordinator of mitochrondrial and nuclear transcription for cytochrome oxidase in neurons and assisting in the regulation of rpL32 gene transcription. Function: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Subunit: Heterotetramer of two alpha and two beta subunits. Interacts with HCFC1, causing repression of transcriptional activity. Subcellular Location: Nucleus. Similarity: Contains 5 ANK repeats. SWISS: Q06547 Gene ID: 2553 Database links: Entrez Gene: 2553 Human Entrez Gene: 14391 Mouse Omim: 600610 Human SwissProt: Q06547 Human SwissProt: Q00420 Mouse Unigene: 654350 Human Unigene: 293266 Mouse Unigene: 61149 Rat |
| 产品图片 |
Protein: A549(human) lysates at 30ug;
Primary: rabbit Anti-GABPB2 (bs-13262R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:42 kD
Observed band size:42 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
