| 产品编号 | bs-13266R |
| 英文名称 | Galectin 10 Rabbit pAb |
| 中文名称 | 半乳糖凝集素10抗体 |
| 别 名 | BSF3; Charcot Leyden crystal protein; Charcot-Leyden crystal protein; CLC; EC 3.1.1.5; Eosinophil lysophospholipase; Gal-10; Galectin-10; LGALS10; LGALS10A; LPPL HUMAN; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659. |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 16 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Galectin 10: 51-142/142 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Charcot-Leyden crystals are endogenous hexagonal bipyramidal crystals present in human tissues and secretions. Presence of Charcot-Leyden crystals correlates with the increased numbers of peripheral blood or tissue eosinophils that occurs with parasitic and allergic processes. Galectin-10, also referred to as Charcot-Leyden crystal (CLC) protein, singularly makes up these crystals. Galectin-10, a member of the galectin family of beta-Galactoside binding proteins that bind to S-type animal lectins, is expressed solely in eosinophilic and basophilic leukocytes. Galectin-10 may possess carbohydrate or IgE-binding activities, and it plays a functional role in the biology of inflammation. Expression of galectin-10 is transcriptionally induced by butyric acid. Function: May have both lysophospholipase and carbohydrate-binding activities. Subcellular Location: Cytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell. Tissue Specificity: Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Similarity: Contains 1 galectin domain. SWISS: Q05315 Gene ID: 1178 Database links: Entrez Gene: 1178 Human Omim: 153310 Human SwissProt: Q05315 Human Unigene: 889 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 10) Polyclonal Antibody, Unconjugated (bs-13266R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 10) Polyclonal Antibody, Unconjugated (bs-13266R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galectin 10 Polyclonal Antibody, Unconjugated(bs-13266R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
