| 产品编号 | bs-13282R |
| 英文名称 | GAPDHS Rabbit pAb |
| 中文名称 | 精子3-磷酸甘油醛脱氢酶-2抗体 |
| 别 名 | GAPDH2; GAPD2; GAPDH 2; GAPDH 2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Sperm |
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Specific References (4) | bs-13282R has been referenced in 4 publications.
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[IF=6.63] Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17 WB ; Mouse. 222
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[IF=4.068] Ming Li. et al. ADAMTS12, a novel prognostic predictor, promotes cell proliferation, migration and invasion in head and neck squamous cell carcinoma. ORAL DIS. 2022 Oct;: WB ; Human. 222
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[IF=2.09] Zhang, Jizheng, Hua Zhang, and Tingting Zi. "Overexpression of microRNA-141 relieves chronic constriction injury-induced neuropathic pain via targeting high-mobility group box 1." International Journal of Molecular Medicine. WB ; Rat. 222
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[IF=1.918] Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1–e928619-10 WB ;
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| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GAPDHS: 251-350/408 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein belonging to theglyceraldehyde-3-phosphate dehydrogenase family of enzymes thatplay an important role in carbohydrate metabolism. Like its somaticcell counterpart, this sperm-specific enzyme functions in anicotinamide adenine dinucleotide-dependent manner to removehydrogen and add phosphate to glyceraldehyde 3-phosphate to form1,3-diphosphoglycerate. During spermiogenesis, this enzyme may playan important role in regulating the switch between differentenergy-producing pathways, and it is required for sperm motilityand male fertility. [provided by RefSeq, Jul 2008]. Function: May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. Subunit: Homotetramer. Interacts with ARRB2; the interaction isdetected in the nucleus upon OR1D2 stimulation. Subcellular Location: Cytoplasm. Tissue Specificity: Testis specific. Similarity: Belongs to the glyceraldehyde-3-phosphatedehydrogenase family. SWISS: O14556 Gene ID: 26330 Database links: Entrez Gene: 26330 Human Entrez Gene: 14447 Mouse Omim: 609169 Human SwissProt: O14556 Human SwissProt: Q64467 Mouse |
| 产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-GAPDHS (bs-13282R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated(bs-13282R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
