| 产品编号 | bs-13297R |
| 英文名称 | GBA2 Rabbit pAb |
| 中文名称 | β葡萄糖苷酶2抗体 |
| 别 名 | AD035; beta glucocerebrosidase 2; beta glucosidase 2; DKFZp762K054; glucosidase, beta(bile acid) 2; Glucosylceramidase 2; KIAA1605; MGC16895; Non lysosomal glucosylceramidase; GBA2_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 发育生物学 信号转导 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Rat, Chicken, Dog, Pig, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 105 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GBA2: 601-700/927 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Beta-glucosidase 2, also known as non-lysosomal glucosylceramidase (NLGase), Glucosylceramidase 2, GBA2 or AD035, is a 927 amino acid non-lysosomal glucosylceramidase that catalyzes glucosylceramide into ceramide and free glucose, and is suggested to play a role in carbohydrate transport and metabolism. A single-pass membrane protein, Beta-glucosidase 2 exists as three alternatively spliced isoforms that are widely expressed but found at highest levels in placenta, kidney, brain, skeletal muscle, kidney and heart, with low levels found in liver. Beta-glucosidase 2 activity has been linked to sphingomyelin generation and prevention of glycolipid accumulation. The gene encoding Beta-glucosidase 2 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. Function: GBA2 is a non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide. It is involved in sphingomyelin generation and prevention of glycolipid accumulation. GBA2 may also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo. Subcellular Location: Cell membrane; Single-pass membrane protein. Note=Not localized to lipid rafts. Tissue Specificity: Widely expressed. Highly expressed in brain, heart, skeletal muscle, kidney and placenta and expressed at lower level in liver. Similarity: Belongs to the non-lysosomal glucosylceramidase family. SWISS: Q9HCG7 Gene ID: 57704 Database links: Entrez Gene: 57704 Human Entrez Gene: 230101 Mouse GenBank: NM_020944.2 Human Omim: 609471 Human SwissProt: Q9HCG7 Human SwissProt: Q69ZF3 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
