| 产品编号 | bs-13362R |
| 英文名称 | GJA10 Rabbit pAb |
| 中文名称 | 间隙连接蛋白10抗体 |
| 别 名 | Gap junction alpha 10; Connexin-62; Cx62; CXA10_HUMAN; Gap junction alpha 10; Gap junction alpha 10 protein; Gap junction alpha-10 protein; Gap junction protein alpha 10 59kDa; GJA10; MGC50985; RP11-63K6.6; Gap junction alpha 10; Connexin 58; Connexin 59; Connexin-62; CX58; CX59; Cx62; CXA10_HUMAN; Gap junction alpha 10; Gap junction alpha 10 protein; Gap junction alpha 9 protein; Gap junction alpha-10 protein; Gap junction protein alpha 10 59kDa; Gap junction protein alpha 9 59kDa; GJA10; MGC50985. |
| 研究领域 | 心血管 细胞生物 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Chicken, Cow, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GJA10: 151-250/543 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Connexins, such as GJA10, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008]. Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Involved in tracer coupling between horizontal cells of the retina. May play a role in the regulation of horizontal cell patterning. Subunit: A connexon is composed of a hexamer of connexins. Subcellular Location: Cell membrane. Cell junction; gap junction. Tissue Specificity: Expressed in skeletal muscle and heart. Similarity: Belongs to the connexin family. Alpha-type (group II) subfamily. Database links: UniProtKB/Swiss-Prot: Q969M2.1 Entrez Gene: 84694 Human Omim: 611924 Human SwissProt: P57773 Human SwissProt: Q969M2 Human Unigene: 334499 Human Unigene: 632402 Human
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| 产品图片 |
Tissue/cell: Rat skeletal muscle; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GJA10 Polyclonal Antibody, Unconjugated(bs-13362R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
